PetakaG3 2% Oxygen (Autonomous hypoxia chamber for cell culture)
Celartia introduces Petaka G3 the most physiologic hypoxia chamber in the world, mimicking as close as possible, how tissue cells in an organism are exposed to oxygen availability deficit. Inside Celartia’s hypoxia chambers cells are exposed to a progressive reduction of oxygen availability from 45 mmHg down to the lowest oxygen tensions recorded and published on living vertebrates: 13.32 mmHg.
Outstandingly, Celartia’s Petaka G3 hypoxia chambers achieve this in open atmosphere, inside a regular incubator, without accessory gas canisters, electronic sensors, feedback electronics or power supply. Celartia’s hypoxia chamber’s manipulation is even easier than regular multi-well plates, and does not need special training to use them at top level of efficiency.
Celartia’s hypoxia chambers Petaka G3 are completely autonomous. Without hypoxia disturbances Celartia’s hypoxia chambers allow for bench top and open regular atmosphere use: 1. Continuous measurement of the oxygen reserve in the media. 2. Permanent evaluation of cellular oxygen consumption per cell and time. 3. Cell culture exposure to hadron beams, electron beams and electromagnetic radiations maintaining the hypoxia level. 4. Celartia’s hypoxia chambers are closed and highly protected against contamination 5. Infinite media changes, sampling and special substances (and investigative drugs) additions to the media are performed in regular laminar flow hood. 6. Laboratory bench Microscopy observations and time lapse video recording with regular microscopes, without glove boxes or any other ambient controlling devices 7. Can be incubated in any sort of temperature controlled device such as plain incubators, CO2 incubators, heat blocks or even water baths. 8. Celartia’s hypoxia chambers can be piled in arrays with efficient space and energy management 9. Celartia’s hypoxia chambers are disposable.
The scientific community agrees that mammal tissue hypoxia is a progressive onset status produced when the red blood cells can’t replace efficiently the oxygen of the interstitial media consumed by the tissue cells. This is exactly how Celartia’s hypoxia chambers regulate the hypoxia levels: in close interaction with the living cells. The proprietary micro-channel respiratory system incorporated in the device maintains under control the oxygen exchange, stabilizing the minimal level according to the type and number of cultured cells.
The discovery of the stress response to the oxygen deficit, specifically the cell response through the Hypoxia Induced Factor (HIF) and collaterals, opened a broad field of research in cell biology. As a consequence, new technologies were applied to produce environments where culturing cells with limited oxygen availability were possible. The straight procedure used in industry has been the production of hermetic boxes (hypoxia chambers) filled and/or purged with the required gas mixture. Inside these boxes traditional cell culture devices (flasks, bags and dishes) are installed and the whole assembly need to be introduced in incubators to achieve the required temperature.
This layer over layer arrangement implies many difficulties: 1. Restricted accessibility to the cell culture device impairing: a. Culture observation b. Media changes c. Adding supplements to the media
2. Gas composition Instability, due to: a. The box needs to be opened for many required cell culture management operations. b. Traumatic steps from normoxia to hypoxia in minutes c. Gas decompression effect inside the cells d. Uncontrolled gas diffusion through box materials and maintenance requirements
3. Only limited stepwise of hypoxia. There is no continuous transition from normoxia to hypoxia. 4. Exaggerated waste of space (volume) 5. Requirement of gas canisters as part of the assembly
Only a few highly sophisticated glove boxes available include a control unit program that permit gradual hypoxia in the chamber, where the cell culture devices are incubated; however, these complex devices are enormous, need great user training, are time consuming, need huge gas canisters, considerable power supply and laboratory space.
All of these restrictions and limitations are beat by Petaka G3 hypoxia chamber technology.